Molecular characterization of human reservoirs potentially pathogenic Escherichia coli, animal and environmental

Characterization of potentially pathogenic E. coli samples from different origins. Detection of virulence genes using quantitative real time PCR to distinguish the samples intended for isolation of a positive strain. Studies of antibiotic resistance in strains from healthy animals. Assess the genetic diversity of STEC isolates by pulsed field gel electrophoresis (PFGE) and Next Generation Sequencing (NGS). Detection serogroups / serotypes related-genes associated with hemolytic uremic syndrome (HUS). Shigatoxin producing E. coli prevalence studies in healthy animals.